Reagent integration

Integrated dried reagent storage

Lab-on-a-chip devices often require one or more reagents to provide accurate test results. These reagents can be added either as a liquid during the assay or pre-loaded on the device in a dried format. This dried format will then be reconstituted during the assay, thus reducing handling steps for the end-user and ensuring a longer shelf life for the product.

Micronit offers the ability to deposit picolitre spots with high accuracy on your desired substrate using an in-house automated piezo actuated non-contact dispensing system. This technology is ideal for depositing reagents or spotting microarrays on microscope slides, biosensors, membranes, microfluidic devices and many other substrates. Varying droplet volumes allow for transferring common assays into a microfluidic format, thereby reducing the amount of costly sample and reagents required as well as the assay turnaround time. It is possible to accurately deposit the reagents in your desired array on basically any substrate type. Furthermore, the ability to control humidity and temperature allow for controlled drying of reagents for consistent functional results.

Our experts have experience in spotting various reagents including proteins (i.e. enzymes, antibodies), nucleic acids (i.e. oligonucleotides), buffers, nano particles (i.e. beads), sugars and surfactants that allow us to support the development, integration and/or miniaturisation of your assay to a microfluidic format.

Spotting specifications for non-contact low volume liquid dispensing

  • Spotting area up to 80x36cm in size
  • Standard droplets of 360-440pl
  • Additional droplet range extension to 100-800pl
  • Spot dimensions are dependent on reagent and spotting substrate combination
  • Typically: 400pl – Ø 125/150µm | 10nl – Ø ~350µm
  • Spotting accuracy < 5µm
  • Time to spot 1µl < 30s
  • A cooling unit for reagents and target area
  • Controllable humidity

Droplets can be spotted individually, on top of each other, in an array or even overlapping

Customised quality control includes:

  • Target recognition and quality control of the spotted samples
  • Stroboscope camera for visual droplet shape and volume control

Integrated liquid reagent storage

Lab-on-a-chip devices often require one or more liquid reagents or buffers stored on chip. The storage of liquid reagents directly on chip eliminates the need for loading of reagents by conventional pipetting in a manual fashion in the lab. This also allows the use of the exact amount of reagent needed eliminating unnecessary wasting of reagents. We work with frangible seal blisters which can be integrated on chip and allow the releasing of reagents directly inside the microfluidic structures of the chip. Blisters release accurate volumes of reagents or buffer under the simple pressure of a finger or force applied by a mechanical system. PoC devices as such are then ready to be operated directly at the patient site.