Next-generation sequencing

DNA next-generation sequencing

Over the years we have been of service to many customers with the fabrication and delivery of flow cell devices for next-generation sequencing (NGS). Today our customers, varying from ambitious start-ups to some of world’s largest analytical and diagnostic instrument manufacturers, can rely on a proven track record in Next-generation sequencing devices.
At the request of our customers, we offer customised flow cells to address their specific sequencing needs. Optical grade, high-quality glass flow cells enable a versatile platform for performing a broad variety of sequencing tests. 

To meet our customers’ needs and requirements, we designed various functionalities for integration in consumables for next-generation sequencing. One can think of valves, electrodes and sensors.  But the application of coatings or nanopatterning is also possible. 

In the latter case, we have developed a flow cell with nanopatterned features integrated into its microfluidic channels. These nanosized wells enable the manufacturing of next-generation sequencing products with higher data density through patterned clustering of DNA fragments. This particular flow cell consists of an optical grade glass top layer and a silicon-based bottom layer. These layers are bonded through a patternable adhesive bonding process providing a solid seal with secured flow channels.

Micronit nanopatterned flowcell.jpg nanopattern in glass-silicon flow cell.jpg

Opportunities for next-generation sequencing devices

  • Extensive choice of materials: Glass, polymer, silicon and hybrid (for example an optical top layer and a CMOS silicon-based bottom layer).
  • Multiple bonding solutions: Laser-assisted (RT) bonding, patternable adhesive bonding (e.g. glass-CMOS wafers), eutectic bonding, anodic bonding and direct/fusion bonding.
  • Optical grade accuracy: Highly accurate, optical grade flow cells with a surface roughness (Ra) of less than 1 nm to ensure full transparency.