Capillary Electrophoresis

      Injection of a fluorescent fluid plug into a
      50 µm wide separation channel (T-junction)  

Capillary electrophoresis is a separation technique that has been very successful in the analysis of DNA fragments and other biomolecules.

CE Process
In capillary electrophoresis, small amounts of fluids are injected into a separation channel by using plug injection. Substances are separated based on their electrophoretic mobility, which is proportional to their charge to size ratio.

Advantages
Capillary electrophoresis with microfluidic chips offers advantages over traditional CE. Having an integrated injection structure enables injection of much smaller volumes than would be possible in standard CE. This means the separation channels can be very short (a few centimeters) for certain applications, which results in much faster analysis. An additional advantage of using chips is that the detection can be integrated in the chip.



Capillary Electrophoresis ChipsCapillary Electrophoresis Chips
Different CE chips with or without integrated electrodes.




 

Microfluidic CE Kit EOF 9015Capillary Electrophoresis Kit (EOF 9015) 
Microfluidic tool kit for on-chip capillary electrophoresis.




 

CE Chips and TricksOn-chip Electrophoresis Devices: Do's, Dont's and Dooms
Tips and tricks for working with on-chip capillary electrophoresis (published at the website of RSC's Lab on a Chip magazine).